Aloe vera is an oldest medicinal plant grown worldwide. The first written record about the use of Aloe vera is found on 6,000 years old clay tablets found in Mesopotamia. The plant was believed to be originated from African continent specifically in Egypt (Daodu, 2000). Aloe vera (Aloe barbadensis) is a perennial, drought resisting succulent plant belongs to the family Liliaceae. The present investigations were carried out on different aspects like isolation, purification & identification of disease through fungicides and botanicals, disease caused by Fusarium oxysporum, leaf rot disease of Aloe barbadensis. The Fusarium oxysporum isolated from infected Aloe vera leaves on PDA medium. The colonies of the fungus white, brown in colour. The pathogen causing leaf rot in Aloe vera was isolated on modified Potato Dextrose Agar (PDA) medium after surface sterilization of infected leaf tissue. Following mycelial growth, purification was done using the hyphal tip method, and the culture was maintained on PDA slants. Based on cultural and morphological characteristics, the pathogen was identified as Fusarium oxysporum. The colony appeared white and fluffy; microscopic examination revealed septate, hyaline mycelium, with slightly curved, coma-shaped microconidia (0–1 septa), strongly curved macroconidia (1–4 septa), and oval to globose chlamydospores, solitary or in chains. These features matched descriptions by Booth (1971), Singh (2006), Ji et al. (2007), Chavan and Korekar (2011), and Hirooka et al. (2007). The management of Fusarium oxysporum, leaf rot disease of Aloe barbadensis was done earlier mostly through fungicides to minimizing the yield losses. But fungicides caused many problems in our eco-system. Despite some progress made towards development of Integrated Disease Management. There is a need to exploitation some other alternative tools viz., use of plant extracts and inter-cropping in disease management.

The present investigation entitled “A Case Study on the Isolation, Purification, Identification & maintenance of the Pathogen Causing Leaf Rot in Aloe vera (Aloe barbadensis Miller)’’ was carried out in the Department of Plant Pathology, Horticulture farm, Narendra Deva University of Agriculture and Technology, Narendra Nagar, Kumarganj, Faizabad (U.P.). Some diseases have been reported on Aloe barbadensis that can lead to loss in quality of leaves, mucilage, gel content and yield, if not managed properly in time. Aloe vera leaf rot is an important disease caused by Fusarium oxysporum which causes quantitative as well as qualitative losses. Keeping in view, the importance of Aloe vera and its medicinal value and visualizing the seriousness of the disease, the present studies were carried out to know as first is the Isolation, Purification, Identification second is Screening of germplasms, third is Epidemiological studies and fourth is management of disease through fungicides and botanicals. The salient findings of the present investigation are as follows: The pathogen was isolated on Modified Potato-Dextrose-Agar medium in Petri plate by transferring them after surface sterilization after the mycelial growth, the fungus was purified through hyphal tip culture method. Subsequently, the culture was maintained on PDA slant for further studies. The pathogen under study was identified as Fusarium oxysporum on the basis of its cultural and morphological characters. Settled spores were separated out from each other, selected under the microscope and enriched with the help of dummy culture in petri-plates. They were lifted along with agar blocks and transferred to petriplates containing sterilized 2% PDA. After proper growth of fungus obtained by single spore culture regular sub-culturing was done to check contamination till pure cultures were obtained. These cultures were sub-cultured at monthly intervals and maintained on PDA slant under refrigeration at 6 to 8 0C temperature for further studies. Chemical control the maximum per cent disease control were recorded in treatment T3= Propiconazole 25 EC @ 0.25% PDC (65.20, 65.71 and pooled 65.46) followed by T2= Mancozeb 75 WP@ 0.25% PDC (61.05, 62.28 and pooled 61.67), and T1= Carbendazim 50 WP@ 0.25% PDC (59.36, 60.28 and pooled 59.82) during 2014 and 2015. Among the botanicals maximum per cent disease control were recorded T4= Neem leaf extract @ 5% PDC (58.26, 59.16 and pooled 58.71) followed by T5= Garlic bulb extract @ 5% PDC 51.81, 51.79 and pooled 51.80) and T6= Tulsi leaf extract @ 5% PDC 44.99, 45.84 and pooled 45.41) during both the years 2014 and 2015, respectively.

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